The Toronto Wildlife Centre has grown to become Canada's busiest wildlife centre. Since opening in 1993, hundreds of thousands of people have received help on TWC's Wildlife Hotline, and over 74,000 wild animals representing over 270 different species have been admitted for care.

TWC's work is carried out by a small group of highly skilled paid staff, and several hundred dedicated volunteers working in all areas of the centre, from wildlife care to administration. They work in cooperation with numerous agencies including the Ontario Ministry of Natural Resources, the Canadian Cooperative Wildlife Health Centre, the Toronto Zoo, the Ontario Veterinary College, many branches of the OSPCA, local animal services agencies, the municipal police and the OPP, as well as other non-profit organizations such as the Owl Foundation, the Canadian Peregrine Foundation, Animal Alliance of Canada and Fatal Light Awareness Program.

Funding from The Gordon and Patricia Gray Animal Welfare Foundation was used to help purchase medical equipment much needed to help injured and sick raptors.